Helps Animal Digest Feed Additive Cellulase CAS NO.9012-54-8

Cellulase
CAS: 9012-54-8
EINECS: 232-734-4
Density 1.2 g/mL at 25 ° C
Storage temp. 2-8° C
Form powder
Color white
Usage As feed additives, helps animal digest and absorp feed. It will Hydrolysis β -1, 4- glucan in cellulose polysaccharides into β -Dextrin.

Synonyms:
Celluase;
Cellulasefromtrichodermalongibrachia-tum;
Fungalcellulase;
1, 4-[1, 3: 1, 4]-BETA-D-GLUCAN;
1, 4-[1, 3: 1, 4]-BETA-D-GLUCAN 4-GLUCANO-HYDROLASE;
3.2.1.4; IUB: 3.2.1.4;
MEICELASE

Product Categories:
Biochemistry;
Enzyme;
Glycohydrolase;
Hydrolase;
3.2. X. X Glycosidases;
3. X. X. X Hydrolases;
Application Index;
Carbohydrate hydrolysis&PTM analysis;
Carbohydrate HydrolysisEnzyme Class Index

Sources of cellulase is very extensive, insects, mollusks, protozoa, bacteria, actinomycetes, fungi can produce cellulase.
1. Bacteria: Bacteria produce lower amounts of cellulase, mainly EG, no activity of most of the crystalline cellulose, and the resulting enzyme is adsorbed on intracellular enzymes or bacterial wall, few can be secreted into the extracellular, increasing the difficulty of extraction and purification, the actual rarely used in industry. More current research is cellulose slime mold species, genus Dictyostelium spore fibers and fibers of the genus.
2. Actinomycetes: Few studies of actinomycetes. Mycobacteria and actinomycetes hardly original production of cellulase or a very low yield, yield slightly higher spin mainly black and red silk actinomycetes, rose actinomycetes, fiber actinomycetes and white roses actinomycetes.
3. Fungi: Currently microorganisms for the production of cellulase are mostly fungi, more research has Trichoderma, Aspergillus, Rhizopus and paint spots of mold. They can produce large amounts of cellulase production especially in Trichoderma majority. Lee and Trichoderma viride and other Trichoderma activity is higher species. Fungi produce three types of cellulase secreted into the cell, generally do not aggregate to form a multi-enzyme complex, but the occurrence of strong mutual synergy. There has been formulated Trichoderma, Aspergillus niger, Fusarium, and Paecilomyces and Penicillium decumbens and other cellulases.
4. Yeast: Industrial production of cellulase due to the need for greater production of the enzyme, exocrine and heat resistance. Recently, more research on cellulase gene cloning. Although almost all of the cloned gene can be expressed in E. Coli, but because of extraction difficulties and low expression levels, will now turn to study gene expression in eukaryotic expression system, the current application of more yeast expression system. Yeast does not produce toxin, the expression of cellulase genes with which the product of highly glycosylated, after correctly processed directly secreted into the medium can be modified to express high levels. CHBII such as yeast expression yield EGI up to 100mg / L or more, and having a normal biological activity.

Cellulase enzymes that catalyze the hydrolysis of cellulose to produce glucose degradation, and a low degree of fiber is a generic term of a group of enzymes, including the endoglucanase enzyme, exo-glucanase enzymes and cellobiase three main components, which not a single one enzyme, but rather a multi-component enzyme system from synergies. Cellulase is an off-white powder or liquid. 1906 Seilliere cellulases found in snail digestive juice. The cellulase enzyme may be β -1, 4- glucosidic bond hydrolysis of fiber, to form soluble polymers and D- glucose. Depending on the source of the enzyme, its lowest relative molecular mass of 5000, up to 400, 000. The optimum pH value of 4.0 to 5.0. Optimum temperature 40 ~ 60 º C. Certain metal ions Mg2 +, Cl2 + and neutral salts can make this enzyme activation, and some metal ions Ag2 +, Cu2 +, Mn2 +, Hg2 + and dye allows this enzyme inactivation.